Gene Mapping Goes from FISH to Surfing the Net.- Linkage Analysis of Genetic Disorders.- Gene Ordering and Localization by Linkage Analysis.- Gene Mapping Using Somatic Cell Hybrids.- Gene Mapping by FISH.- Probe Ordering and Distancing by FISH.- Physical Mapping by Pulsed-Field Gel Electrophoresis.- Construction and Use of YAC Contigs in Disease Regions.- Construction and Use of Cosmid Contigs.- Use of Dinucleotide Polymorphism Analyses in Physical Mapping.- Mapping Expressed Sequence Tags (ESTs) by Multiplexing PCR Reactions from Hybrid Cell Panels and Detecting Fluorescently Labeled Products.- Gene Isolation by Exon Trapping.- Isolation of Coding Sequences from Genomic Regions Using Direct Selection.- Isolation of cDNAs Using the YAC Hybridization Screen Method.- Detection and Isolation of Differentially Expressed Genes by Differential Display.- Chemical Crosslinking Subtraction (CCLS).- Gene Mapping and Isolation.- Software for Genetic Linkage Analysis.- Exon Detection by Similarity Searches.
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Zustand: New. This text is designed for those wishing to learn the techniques applied by experts in gene mapping. It includes: the localization of genes by FISH and linkage analysis; gene isolation by YAC hybridization; direct selection; and exon trapping and differential display. Series: Methods in Molecular Biology. Num Pages: 318 pages, biography. BIC Classification: PSAK; PSF. Category: (P) Professional & Vocational; (UP) Postgraduate, Research & Scholarly; (UU) Undergraduate. Dimension: 229 x 152 x 18. Weight in Grams: 1080. . 1996. Paperback. . . . . Books ship from the US and Ireland. Artikel-Nr. V9780896033825
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