ism (i. e. , Saccharomyces carlsbergensis, or brewer's yeast) and one of its corresponding enzymes. The experiments on this organism and enzyme are not limited to the materials suggested and can be easily adapted to the desired technical level and available budget. Similarly, the subse quent cloning experiments suggest that use of particular vectors and strains, but, as indicated, alternative materials can be used to success fully perform the laboratory exercises. We would like to thank the corporate sponsors of the Biotechnology Training Institute for providing the materials and expertise for the devel opment of our programs, and thus for the materials in this manual. These sponsors include: • Barnsteadffhermolyne, Dubuque, IA • Beckman Instruments, Somerset, NJ • Bio-Rad Laboratories, Hercules, CA • Boehringer Mannheim Corporation, Indianapolis, IN • Coming Costar Corporation, Cambridge, MA • FMC BioProducts, Rockland, ME • Kodak Laboratory Products, New Haven, CT • Labconco, Kansas City, MO • MJ Research, Cambridge, MA • Olympus Instruments, Lake Success, NY • Pharmacia Biotech, Piscataway, NJ • Savant, Inc. , Farmingdale, NY • VWR Scientific, Philadelphia, P A We would also like to thank the following individuals for their input, comments, and suggestions: Tom Slyker, Bernie Janoson, Steven Piccoli, John Ford,JeffGarelik, Yanan Tian, and Douglas Beecher. Special thanks to Alan Williams for his critique of the chromatography experiments and Shannon Gentile for her work in the laboratory. We would especial ly like to thank Maryann Burden for her comments and encouragement.
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ism (i. e. , Saccharomyces carlsbergensis, or brewer's yeast) and one of its corresponding enzymes. The experiments on this organism and enzyme are not limited to the materials suggested and can be easily adapted to the desired technical level and available budget. Similarly, the subse quent cloning experiments suggest that use of particular vectors and strains, but, as indicated, alternative materials can be used to success fully perform the laboratory exercises. We would like to thank the corporate sponsors of the Biotechnology Training Institute for providing the materials and expertise for the devel opment of our programs, and thus for the materials in this manual. These sponsors include: · Barnsteadffhermolyne, Dubuque, IA · Beckman Instruments, Somerset, NJ · Bio-Rad Laboratories, Hercules, CA · Boehringer Mannheim Corporation, Indianapolis, IN · Coming Costar Corporation, Cambridge, MA · FMC BioProducts, Rockland, ME · Kodak Laboratory Products, New Haven, CT · Labconco, Kansas City, MO · MJ Research, Cambridge, MA · Olympus Instruments, Lake Success, NY · Pharmacia Biotech, Piscataway, NJ · Savant, Inc. , Farmingdale, NY · VWR Scientific, Philadelphia, P A We would also like to thank the following individuals for their input, comments, and suggestions: Tom Slyker, Bernie Janoson, Steven Piccoli, John Ford,JeffGarelik, Yanan Tian, and Douglas Beecher. Special thanks to Alan Williams for his critique of the chromatography experiments and Shannon Gentile for her work in the laboratory. We would especial ly like to thank Maryann Burden for her comments and encouragement.
This is a manual which aims to give students a chance to explore the process and techniques of characterizing and purifying a protein, and of subsequent cloning of the associated gene. Each chapter emphasizes the process of discovery, and the strategies and rationale for each experiment. To minimize cost and avoid extensive laboratory preparation, the experiments in this manual use readily available materials and organisms, the bacterium Escherichia coli and the yeast Saccharomyces carlsbergensis. Laboratory topics include: microbiological methods (handling, storage, media); organism growth and protein production; enzyme and protein essays (specific activity); batch purification of proteins; gel filtration and ion exchange chromatographies; genomic and plasmid DNA isolation; and DNA sequencing and computer analysis.
„Über diesen Titel“ kann sich auf eine andere Ausgabe dieses Titels beziehen.
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Taschenbuch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - ism (i. e. , Saccharomyces carlsbergensis, or brewer's yeast) and one of its corresponding enzymes. The experiments on this organism and enzyme are not limited to the materials suggested and can be easily adapted to the desired technical level and available budget. Similarly, the subse quent cloning experiments suggest that use of particular vectors and strains, but, as indicated, alternative materials can be used to success fully perform the laboratory exercises. We would like to thank the corporate sponsors of the Biotechnology Training Institute for providing the materials and expertise for the devel opment of our programs, and thus for the materials in this manual. These sponsors include: Barnsteadffhermolyne, Dubuque, IA Beckman Instruments, Somerset, NJ Bio-Rad Laboratories, Hercules, CA Boehringer Mannheim Corporation, Indianapolis, IN Coming Costar Corporation, Cambridge, MA FMC BioProducts, Rockland, ME Kodak Laboratory Products, New Haven, CT Labconco, Kansas City, MO MJ Research, Cambridge, MA Olympus Instruments, Lake Success, NY Pharmacia Biotech, Piscataway, NJ Savant, Inc. , Farmingdale, NY VWR Scientific, Philadelphia, P A We would also like to thank the following individuals for their input, comments, and suggestions: Tom Slyker, Bernie Janoson, Steven Piccoli, John Ford,JeffGarelik, Yanan Tian, and Douglas Beecher. Special thanks to Alan Williams for his critique of the chromatography experiments and Shannon Gentile for her work in the laboratory. We would especial ly like to thank Maryann Burden for her comments and encouragement. Artikel-Nr. 9780817638436
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