High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.
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Since its infancy in the late 1960s and early 1970s, the use of high perfor mance liquid chromatography (HPLC, or LC as it is increasingly becom ing known) has expanded rapidly. Today, it is a highly popular analytical technique which is extensively used in many fields of activity. Not surpris ingly, it has been the inspiration for much scientific literature, including a significant number of books. These books have taken various forms, such as practical handbooks, modified short-term manuals, reports on selected topics, theoretical treatises and even attempts at comprehensive works. In almost every way, HPLC may be regarded as a mature technique. However, there is one sense in which it is still developing. Only recently has its importance been acknowledged by accepting it as a subject worthy of inclusion in undergraduate science courses. The aim of this book is therefore to support the newly acquired status of HPLC by providing a presentation of the technique primarily aimed at undergraduate students on courses containing a significant component of analytical science. The intention has been to come up with a different slant to that already avail able, characterised by an emphasis on understanding. Why is HPLC so useful? When should it be used? What is the reason for certain practices? Most importantly, how does HPLC work? The responsibility to deliver a thorough coverage of theory has not been shirked.
High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC.
Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.
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