Excerpt from Stain Technique
Stain Technique is designed to provide concise instruction in the correct usage of biological stains by introducing the chemistry of color precipitates, the arrangement of stains into a few classes depending on their manner of application, and the outline for stain ing with combinations of two, three, or four dyes. The results of numerous tests with each dye indicate the action of acids, alkalies, and salts; the composition of effective stains; the influence of re agents ou the fastness of color; the affinity for tissues of algae, fungi, mosses, vascular plants, invertebrate and vertebrate ani mals; and the combination of stains which provide contrasting colors. Because most of the experimental products are of nega tive value, many of the dyes merit only a brief statement. A few superior and distinctive coal-tar dyes are worthy of admission into standard methods. Hence, the fundamental plan of this textbook is extended to include those phases which assist the student in a practical application of stain technique.
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