Fundamentals of Light Microscopy and Electronic Imaging, Second Edition - Hardcover

This should be provided to all beginning graduate students entering microscopy labs. It describes the complicated hardware of the system, while also explaining the physics principles of microscopy on a simplistic level for basic biologists. The authors achieve a perfect balance of theory and methods. (Doody s, 15 November 2013) It should be particularly useful to researchers getting started in the field of microscopy as well as seasoned professionals. Summing Up: Highly recommended. Graduate students, researchers/faculty, and professionals/practitioners. (Choice, 1 October 2013) In summary, Fundamentals of Light Microscopy, Second Edition is a recommended starting point for the novice in microscopy and electronic imaging. (Journal of Biomedical Optics, 1 February 2013)

Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy