Über die Autorin bzw. den Autor

Martin E. Schimpf is the author of Field-Flow Fractionation Handbook, published by Wiley.

Auszug. © Genehmigter Nachdruck. Alle Rechte vorbehalten.

Field-Flow Fractionation Handbook

John Wiley & Sons

Copyright © 2000 Martin E. Schimpf
All right reserved.
ISBN: 978-0-471-18430-0

Contents

Preface.................................................................................................................................................ixContributors............................................................................................................................................xiList of Symbols.........................................................................................................................................xvSolvent Properties......................................................................................................................................xixI. PRINCIPLES AND THEORY................................................................................................................................11. The Field-Flow Fractionation Family: Underlying Principles J. Calvin Giddings.......................................................................32. Retention-Normal Mode Mark R. Schure, Martin E. Schimpf, and Paul D. Schettler......................................................................313. Band Broadening and Plate Height Joe M. Davis.......................................................................................................494. Resolution and Fractionating Power Martin E. Schimpf................................................................................................715. Steric Field-Flow Fractionation and the Steric Transition Karin D. Caldwell.........................................................................796. Optimization Martin E. Schimpf.......................................................................................................................957. Physicochemical Measurements and Distributions from Field-Flow Fractionation Francesco Dondi and Michel Martin......................................1038. Sedimentation FFF Methodology: Insights through Computer Simulations Mark R. Schure, Karin D. Caldwell, and Bhajendra N. Barman.....................1339. Programmed Field-Flow Fractionation: Retention P. Stephen Williams..................................................................................14510. Programmed Field-Flow Fractionation: Fractionating Power and Optimization P. Stephen Williams......................................................167II. TECHNIQUES AND INSTRUMENTATIONS.....................................................................................................................18311. Experimental Field-Flow Fractionation: Overview Martin E. Schimpf..................................................................................18512. Sample Preparation and Choice of Carrier Liquid in Field-Flow Fractionation Bhajendra N. Barman and Myeong Hee Moon................................18913. Experimental Field-Flow Fractionation: Practices and Precautions Myeong Hee Moon and Marcus N. Myers...............................................19914. Ancillary Equipment Marcus N. Myers, Larry E. Oppenheimer, and Martin E. Schimpf...................................................................21315. Sedimentation Field-Flow Fractionation Myeong Hee Moon.............................................................................................22516. Thermal Field-Flow Fractionation Martin E. Schimpf.................................................................................................23917. Flow Field-Flow Fractionation S. Kim Ratanathanawongs-Williams.....................................................................................25718. Asymmetrical Flow Field-Flow Fractionation Karl-Gustav Wahlund.....................................................................................27919. Electrical Field-Flow Fractionation Karin D. Caldwell..............................................................................................29520. Other Field-Flow Fractionation Techniques James C. Bigelow.........................................................................................31321. Sample Recovery S. Kim Ratanathanawongs-Williams and J. Calvin Giddings............................................................................325III. APPLICATIONS: INDUSTRIAL AND BIOMEDICAL............................................................................................................34522. Latexes and Emulsions Bhajendra N. Barman..........................................................................................................34723. Metal Particles Larry E. Oppenheimer, Stephan Anger, and Karin D. Caldwell.........................................................................36324. Miscellaneous Submicrometer-Sized Particles Bhajendra N. Barman....................................................................................37325. Miscellaneous Particles [greater than or equal to]1 m in Diameter Myeong Hee Moon.................................................................38326. Lipophilic Polymers Seungho Lee....................................................................................................................39727. Synthetic Polymers-Water Soluble Maria Anna Benincasa..............................................................................................40728. Protein Complexes and Lipoproteins Ping Li and Marcia Hansen.......................................................................................43329. Cell Separations Armelle Lucas, Fabienne Lepage, and Phillipe Cardot...............................................................................471IV. APPLICATIONS: ENVIRONMENTAL.........................................................................................................................48730. Overview of Environmental Applications Ronald Beckett..............................................................................................48931. Characterization of Humic Substances Ronald Beckett and Martin E. Schimpf..........................................................................49732. Aquatic Colloids James Ranville and Ronald Beckett.................................................................................................50733. Investigation of Pollutant-Particle Association Deirdre Murphy and Ronald Beckett..................................................................52534. Biological Particles of Environmental Interest Reshmi Sharma and Ronald Beckett....................................................................537Index...................................................................................................................................................561

Preface

Field-flow fractionation (FFF) occupies a unique niche in the field of analytical separations because it is the only technique that is capable of separating materials over the entire colloidal size range (1-1,000 nm) with high resolution. Still, FFF has not enjoyed what can be considered an explosive phase of growth, like those encountered in the development of gas and liquid chromatography. There are many theories as to why this is so, and certainly a combination of factors is responsible. FFF practitioners clearly agree that one hindrance to the widespread use of FFF as a routine tool for sizing macromolecules and colloids stems from its greatest asset. That asset is its versatility, and versatility comes with a price. Thus, even though FFF is applicable to an incredible range of materials, from macromolecules of a few thousand grams per mode or more to particles as large as 100 mm, there is no simple formula for choosing...