Excerpt from Sampling Designs for Estimating the Total Number of Fish in Small Streams
Large first-stage variance for the traditional design results from equal-sized primary units being of unequal habitat quality. Certain selected sections may consist primarily of riffle habitat, whereas others may consist primarily of pool habitat. Because densities of fish usually vary considerably among habitat types, the equal-sized primary unit design results in substantial variation among the numbers of fish in primary units and, thus, in large first-stage variance.
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