How to Genotype Rotavirus Isolates - Softcover

Inhaltsangabe

Viral RNA was extracted from the stool samples collected from the children and analyzed using one step Reverse Transcriptase Polymerase Chain Reaction (RT-PCR ) for genotyping and Agarose Gel Electrophoresis (AGE) for the identification of the different bands of the electrophoretypes of the rotavirus. Analysis results indicated that 16 (22.53%%) out of the 71 children were positive for rotavirus infection while 55 (77.46%%) were negative for rotavirus infection. The rotavirus positive patients were made up of 12 males (68.75%%) and 4 females (25.0%%) while rotavirus negative patients were 32 males (58.18%%) and 23 females (41.81%%). The total number of males with gastroenteritis were 44 (60.56%%) while females were 27 (39.43%%). Rotavirus G2 genotype detected in 10 patients was the most prevalent strain in the hospital followed by G1 genotype found in 6 patients and these were the only rotavirus genotypes detected in the hospital.

Die Inhaltsangabe kann sich auf eine andere Ausgabe dieses Titels beziehen.