Low-Temperature Microscopy and Analysis - Hardcover

Echlin, Patrick

 
9780306439841: Low-Temperature Microscopy and Analysis
Hardcover
ISBN 10: 0306439840 ISBN 13: 9780306439841
Verlag: Springer, 1992

Inhaltsangabe

The frozen-hydrated specimen is the principal element that unifies the subject of low­ temperature microscopy, and frozen-hydrated specimens are what this book is all about. Freezing the sample as quickly as possible and then further preparing the specimen for microscopy or microanalysis, whether still embedded in ice or not: there seem to be as many variations on this theme as there are creative scientists with problems of structure and composition to investigate. Yet all share a body of com­ mon fact and theory upon which their work must be based. Low-Temperature Micros­ copy and Analysis provides, for the first time, a comprehensive treatment of all the elements to which one needs access. What is the appeal behind the use of frozen-hydrated specimens for biological electron microscopy, and why is it so important that such a book should now have been written? If one cannot observe dynamic events as they are in progress, rapid specimen freezing at least offers the possibility to trap structures, organelles, macro­ molecules, or ions and other solutes in a form that is identical to what the native structure was like at the moment of trapping. The pursuit of this ideal becomes all the more necessary in electron microscopy because of the enormous increase in resolution that is available with electron-optical instruments, compared to light­ optical microscopes.

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Reseña del editor

The frozen-hydrated specimen is the principal element that unifies the subject of low­ temperature microscopy, and frozen-hydrated specimens are what this book is all about. Freezing the sample as quickly as possible and then further preparing the specimen for microscopy or microanalysis, whether still embedded in ice or not: there seem to be as many variations on this theme as there are creative scientists with problems of structure and composition to investigate. Yet all share a body of com­ mon fact and theory upon which their work must be based. Low-Temperature Micros­ copy and Analysis provides, for the first time, a comprehensive treatment of all the elements to which one needs access. What is the appeal behind the use of frozen-hydrated specimens for biological electron microscopy, and why is it so important that such a book should now have been written? If one cannot observe dynamic events as they are in progress, rapid specimen freezing at least offers the possibility to trap structures, organelles, macro­ molecules, or ions and other solutes in a form that is identical to what the native structure was like at the moment of trapping. The pursuit of this ideal becomes all the more necessary in electron microscopy because of the enormous increase in resolution that is available with electron-optical instruments, compared to light­ optical microscopes.

Reseña del editor

This comprehensive volume explores the preparation, examination, and analysis of organic, hydrated, and biological specimens using cryomicroscopic techniques. More than 200 illustrations supplement the text.

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Verlag
Springer
Erscheinungsdatum
1992
Sprache
Englisch
ISBN 10 
0306439840
ISBN 13 
9780306439841
Einband
Tapa dura
Anzahl der Seiten
568
Kontakt zum Hersteller
Nicht verfügbar
Verantwortliche Person
CMN Printpool Inh. Mathis Neumann
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Hamburg
Deutschland

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