PCR is now one of the most widely used of basic molecular biology techniques and is an indispensable research tool for the molecular biologist. The basic PCR technique provides the cornerstone for in vitro DNA amplification, upon which further features can be built to expand the range of PCR-based applications. PCR: A Practical Approach Volume 2 is not a revised version of PCR:A Practical Approach, but sets out to address some of the new and exciting applications of PCR including direct sequencing, mRNA quantitation and expression, genomic DNA mapping, and mutation analysis.
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