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Inhaltsangabe
Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end processing, capping, methylation, splicing and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all key steps required for successful experimental investigation. This includes full descriptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNA's and the isolation and analysis of ribonucleoprotein complexes.
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Reseña del editor
Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end processing, capping, methylation, splicing and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all key steps required for successful experimental investigation. This includes full descriptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNA's and the isolation and analysis of ribonucleoprotein complexes.
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- VerlagOxford University Press
- Erscheinungsdatum1994
- ISBN 10 0199633444
- ISBN 13 9780199633449
- EinbandTapa dura
- SpracheEnglisch
- Anzahl der Seiten232
- Kontakt zum HerstellerNicht verfügbar
(Keine Angebote verfügbar)
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