R.N.A. Processing: A Practical Approach, Volume 1 (Paper) (The Practical Approach Series, 135, Band 1) - Softcover

Inhaltsangabe

As post-translational events become increasingly recognized as critical regulation sites of gene expression in eukaryotic cells, intense research interest in R.N.A. processing has rapidly grown. R.N.A. Processing: A Practical Approach, available in two volumes, offers detailed guidance on all major aspects of the subject. Step-by-step protocols from leading laboratories are presented for studying the termination, end-processing, capping, methylation, splicing, and editing of M.R.N.A. . Protocols designed for the study of M.R.N.A. stability and processing of R.R.N.A. and T.R.N.A. are included as well, with full descriptions of the synthesis and purification of R.N.A. substrate for in vitro work, the characterization of specific R.N.A.s, and the isolation and analysis of ribonucleoprotein complexes. Taken together, the two volumes are invaluable laboratory companions to researchers working in M.R.N.A., T.R.N.A., and T.R.N.A. expression. The contributors' lucid explanatory style and comprehensive coverage will be welcomed by both experienced researchers and by students embarking on such studies for the first time.

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