Mammalian Cell Biotechnology: A Practical Approach (Practical Approach Series) (The Practical Approach Series, Band 76) - Softcover

Inhaltsangabe

The development of mammalian cell biotechnology has led to an extensive range of commercially valuable prophylactic, diagnostic, and therapeutic compounds which can now be produced routinely by cell culture. This book details the principles and practical techniques upon which this technological development is based. The ten chapters are designed to cover the basic principles and techniques which can be applied to maximize the productivity of cell culture. Media optimization and the use of serum substitutes or growth factors are explained. Methods of genetic manipulation of cells are explored as well as well as the techniques for maximizing specific protein expression. The design and control of animal fermentation processes and the means of extraction of desired products by downstream processing are explained, as are the strategies for applying these techniques to the production of specific compounds with commercial value. The detailed principles and techniques described in the book should advance the understanding and realization of high productivity from mammalian cell culture.

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