Calcium is arguably the most diverse and widespread of the intracellular second messengers, being involved in many mechanisms of cellular biochemistry, physiology, and pathology, where it plays a key role in signal transduction. Hormones and other extracellular agents bring about many of their effects by causing changes intracellular calcium concentration. This book provides a comprehensive description of state-of-the-art techniques for measuring intracellular calcium concentrations, including protocols for the use of fluorescent, bioluminescent and metallochromic indicators, and various other intracellular probes. Also outlined are methods for controlling and changing the calcium ion concentration in cells, as well as procedures used to study the important proteins involved in the membrane transport and intracellular binding of calcium. All of the chapters provide expert practical guidance in the techniques described and list any problems likely to be encountered. This book will appeal to a wide range of biological and biomedical researchers, particularly those involved in cellular, molecular, and neurobiology.
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'This book is a collection of well-written chapters that focus on practical aspects of cellular Ca2+. The chapters on the methods for measuring [Ca2+]i are informative, and rich with experimental details that only experts working daily with this subject could provide. Lists of reagents required for each procedure are particularly helpful, and represent a direct way to focus the readers' attention on the most relevant issues.'Trends in Cell Biology, Vol. 2, August 1992
'This is a book based on a great deal of practical experience which gives insights into associated problems and is thus extremely useful ... This book has to be strongly recommended for individual purchase due to the wealth of laboratory based protocols, which from the reviewer's brief experience have proved invaluable.'F. McDonald, European Journal of Orthodontics, 15 (1993)
'this book will be useful for someone already working in this area who wishes to gain a working knowledge of the latest experimental procedures on cellular calcium'H.E. Knaggs, Biochemical Education 21(4) 1993
Calcium is arguably the most diverse and widespread of the intracellular second messengers, being involved in many mechanisms of cellular biochemistry, physiology, and pathology, where it plays a key role in signal transduction. Hormones and other extracellular agents bring about many of their effects by causing changes intracellular calcium concentration. This book provides a comprehensive description of state-of-the-art techniques for measuring intracellular calcium concentrations, including protocols for the use of fluorescent, bioluminescent and metallochromic indicators, and various other intracellular probes. Also outlined are methods for controlling and changing the calcium ion concentration in cells, as well as procedures used to study the important proteins involved in the membrane transport and intracellular binding of calcium. All of the chapters provide expert practical guidance in the techniques described and list any problems likely to be encountered. This book will appeal to a wide range of biological and biomedical researchers, particularly those involved in cellular, molecular, and neurobiology.
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