Environmental Microbiology: A Laboratory Manual is designed to meet the diverse requirements of upper division and graduate-level laboratory sessions in environmental microbiology. The experiments introduce students to the activities of various organisms and the analyses used to study them. The book is organized into three thematic sections: Soil Microbiology, Water Microbiology, and Environmental Biotechnology.
The first section includes experiments on soil as a habitat for microorganisms, and introduces the main types of soil microorganisms, how they interact with the soil, and the techniques used in their analysis. Experiments in the second section cover assays of microbial pathogens -- bacteria, viruses, and protozoan parasites -- used in food and water quality control as well as an exercise in applied bioremediation of contaminants in water. The final section on biotechnology includes applications of the polymerase chain reaction (PCR) for the detection of bacteria and the use of enrichment cultures and a computer-based, physiological test bank to isolate and identify a bacterium useful in bioremediation.
Designed for maximum versatility and ease of use for both the student and instructor, each experiment is self-contained and includes theoretical, practical, and pedagogical material.
* Each chapter contains a single laboratory experiment, many of which include illustrations and illustrated procedure schematics
* Experiments are extensively cross-referenced to provide ready access to related information and illustrations found in other experiments
* All of the experiments include lists of materials and equipment as well as media recipes
* Supplementary mathematical, statistical, and chemical analysis information and a comprehensive glossary cross-referenced to the text are found in the appendix
* The entire book has been designed to be versatile and contains perforated pages suitable for use in loose-leaf binders. Wide margins give students ample room for note taking during pre-lab discussions
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Ian L. Pepper is currently the Chair of the Undergraduate Program in Environmental Sciences at the University of Arizona. He was recently named to Who's Who in America for the second year in a row, and in 1994 earned the Researcher of the Year Award in the College of Agriculture at the University of Arizona. In 1994 he was also named as a fellow to the American Society of Agronomy, and he became the Chair of the Soil Biology and Biohemistry Division of the Soil Science Society of America. Pepper is also a Fellow of the American Society of Microbiology, and has been a member of the Editorial Board of Applied and Environmental Microbiology for the past nine years. Pepper has also been the author or co-author of numerous published journal articles.
Dr. Gerba has 30 years experience in the area of wastewater and waste recycling in agriculture. He has been involved in numerous projects on use of soil aquifer treatment for reuse of domestic wastewater for crop production, composting of domestic solid waste, use of wastewater for fish and shellfish aquiculture, surface water pollution and control from animal feeding operations, reuse of sewage sludge on farm land, and use of wetlands for recycling of animal waste. His expertise is in the area of pathogen fate and removal by treatment processes and risk assessment. He has served on numerous local, state, and federal committees and expert workshops for the development of standards and guidelines for the treatment and recycling of wastes to minimize environmental impacts. He was a member of the U. S. Environmental Protection Agency's Science Advisory Board for 8 years. He has organized and participated in workshops on food and water safety throughout the U.S. and Latin America. He has co-authored nine workshop training manuals.Review:
"This is a good, thorough textbook for environmental microbiologists and students. ...the scope covered by this book is very impressive...the case studies are recent and relevent to each chapter...its contents cover a wide range of topics that can be understood easily by someone who is not an expert on the subject."
-J.J. Germida for SOIL SCIENCE (June 2002)
"...the preface itself is a model of clarity explaining what the manual contains and how it should be used."
--H. Humphreys in JOURNAL OF MEDICAL MICROBIOLOGY
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