Colloidal Gold: Principles, Methods, and Applications, Vol. 1 (Colloidal Gold: Principles, Methods & Application)

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9780123339270: Colloidal Gold: Principles, Methods, and Applications, Vol. 1 (Colloidal Gold: Principles, Methods & Application)

Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today.
This universal method is applicable to most microscopical systems including optical microscopy, scanning, transmission and high voltage electron microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection.

Topics covered in Volume 1 include:
* Principles, methods, and applications of colloidal gold methodology in cytochemistry and immunochemistry
* Methods for preparing colloidal gold particles of different sizes
* Protein A-gold, protein G-gold, and lectin-gold techniques
* The use of resins and thin cryosections
* Multiple labeling

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From the Back Cover:

Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today.
Topics covered in Volume 1 include:
* Principles, methods, and applications of colloidal gold methodology in cytochemistry and immunochemistry
* Methods for preparing colloidal gold particles of different sizes
* Protein A-gold, protein G-gold, and lectin-gold techniques
* The use of resins and thin cryosections
* Multiple labeling
Topics covered in Volume 2 include:
* Methodology for the study of antigenicity in neurology, virology, exocytosis, cytoskeleton, and plant tissues
* The use of colloidal gold in conjunction with negative staining, nucleic acid hybridizsation, and darkfield and epipolarization microscopy
* The detection of proteins with colloidal gold
Molecular interactions between gold and proteins
* The roles of tissue processing in colloidal gold methods
* Low temperature methods

About the Author:

Dr. Hayat has published extensively in the fields of microscopy, cytology, immunohistochemistry, immunocytochemistry, and antigen retrieval methods. He is Distinguished Professor, Department of Biological Sciences, Kean University, Union, New Jersey, USA.

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