RNA Processing: A Practical Approach: RNA Processing: a practical approach: Volume II: v.2 (Practical Approach Series) - Softcover

RNA processing is a vital stage in the expression of hereditary information stored in the DNA of genes into the every day activity of cells and organisms. These two volumes provide researchers at all levels with user-friendly experimental protocols for studying all aspects of RNA processing events.

Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end processing, capping, methylation, splicing and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all key steps required for successful experimental investigation. This includes full desciptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNA's and the isolation and analysis of ribonucleoprotein complexes.